We are very happy to announce the live webinar from Leica Microsystems on the topic: Challenges in 3D cell culture and their phenotypic analysis:

In this webinar, you will discover:

• Potential solutions for phenotypic drug screening using 3D-cell models;
• Implications of Rayleigh scattering on 3D-microscopy;
• 3D-image segmentation and marker prediction.

This webinar introduces an overview of the principal challenges in the planning and execution of a phenotypic drug screen and discusses potential solutions and respective drawbacks. 

Please look forwar to:

Speaker: Prof. Dr. Rüdiger Rudolf, Professor of Biosensorics, Mannheim University of Applied Sciences, CeMOS

High Throughput Technology for Hit Generation - The 3^rd Edelris Symposium on Affinity Selection-Mass Spectrometry in Drug Discovery will take place online on May 31 and June 1, 2023.

The Symposium will gather, in a virtual and interactive format, academic and industrial speakers who have made significant contributions to implement AS-MS at different levels of their organisations.

The Symposium will focus on progress and results obtained with this technology in discovering and validating lead-compounds on various challenging targets.

Speaker: Prof. Dr. Carsten Hopf, CeMOS, Mannheim University of Applied Sciences

Titel: MALDI MS-based Cell Assays in Pharmaceutical R&D – a Perspective

This symposium aims to highlight the recent advances and applications of label-free high-throughput cell-based and affinity-selection mass spectrometry (MS) screening assay technologies as an emerging lead discovery strategy. The symposium will bring together leading academic and industry experts to reflect on the opportunities and challenges to exploit state-of-the-art MS-based screening methods to accelerate the discovery of novel starting points for drug discovery.

Speaker

Dr. Sandra Schulz, CeMOS, Mannheim University of Applied Sciences

Cell-based MALDI MS assays for the characterization of enzymatic reactions and transporter uptake

High throughput capable MALDI-TOF MS has become an emerging analytical technology in drug discovery in recent years. Apart from being label-free, this technique is cost effective and represents a robust read-out for several biochemical assays in industrial settings.
Cell-based MALDI MS assays are still underrepresented as they are more complex and difficult to automate and validate. However, they offer the rare possibility of analysing molecular reactions directly in the cellular context without the necessity of elaborate and time consuming separation methods for cellular components. In this talk, approaches and considerations for the development of MALDI MS cell assays in drug discovery are shown. Moreover, three example assays are presented to underline their strength: the profiling of epigenetic histone deacetylase inhibitors, a mechanistic cell-based assay that monitors inhibition of the fatty acid synthase directly in whole cells using the enzyme specific metabolite malonyl-CoA as FASN reaction readout and a compound screen for the identification of inhibitors of the uptake of estrone-3-sulfate through the transport protein OATP2B1.

Prof. Dr. Carsten Hopf, CeMOS, Mannheim University of Applied Sciences

Perspectives of MALDI MS cell assays – from phenotypic screening to miniaturized assays on droplet microarrays

Mechanistic MALDI MS-based cell assays, which measure single analytes in a cellular context, are successfully transitioning into industry. This talk will introduce participants to phenotypic MALDI MS cell. These acquire information on entire molecular signatures comprising arguably hundreds of molecules simultaneously. These can be analyzed using computational tools to reveal drug-response markers or responses biologically relevant stimuli. In addition, this talk will introduce to MALDI MS cell assays on droplet microarrays that utilize MALDI imaging MS as a readout.

REGISTER FOR THIS EVENT

GBM will continue its popular virtual GBM Lunch seminar in 2022:
The seminars take place every Wednesday from 12.30 to 13.30 CET (lectures in English) as a Zoom conference.

Speakers: Stefania-Alexandra Iakab & Rüdiger Rudolf, Hochschule Mannheim, CeMOS - Center for Mass Spectrometry and Optical Spectroscopy
Chair: Carsten Hopf, Hochschule Mannheim
Organizers: Working Group Industry

Please register here: https://gbm-online.de/gbm-lunch.html