Jahr 2020

Fast Quantification Without Conventional Chromatography, the Growing Power of Mass Spectrometry

Journal: ACS Publications

George Gachumi,Randy W. Purves1,2 ,Carsten Hopf3 and Anas El-Aneed1

1College of Pharmacy and Nutrition, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK, Canada, S7N 5E5.

2Centre for Veterinary Drug Residues, Canadian Food Inspection Agency, 116 Veterinary Rd, Saskatoon, SK, Canada, S7N 2R3.

3Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Paul-Wittsack-Strasse 10, 68163 Mannheim, Germany.

Lab‐scale microreactor plant for the study of methylations with liquid chloromethane

Journal: Chemical Engineering Technology

M. Sc. Clarissa V. Benzin1,2,*,Prof. Dr.-Ing. Norbert Kockmann1, Prof. Dr. Thorsten Röder2

1TU Dortmund University, Laboratory of Equipment Design, Emil-Figge-Straße 68, 44227 Dortmund, Germany

2Hochschule Mannheim University of Applied Sciences, Institute of Chemical Process Engineering, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany

Self-optimising processes and real-timeoptimisation of organic syntheses in a microreactor system using Nelder–Mead and design of experiments

Journal: Royal Society of Chemistry

Verena Fath3,*, Norbert Kockmann1, Jürgen Otto2, Thorsten Röder3 

1Department of Biochemical and Chemical Engineering, Equipment Design, TU Dortmund University, Emil-Figge-Str. 70, 44227 Dortmund, Germany

2Institute of Chemical Process Engineering, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany

3Hochschule Mannheim University of Applied Sciences, Institute of Chemical Process Engineering, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany 

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany

Efficient Kinetic Data Acquisition and Model Prediction: Continuous Flow Microreactors, Inline FT-IR Spectroscopy, and Self-modeling Curve Resolution

Journal: Org. Process res. Dev.

Verena Fath1,2, *, Philipp Lau3, Christoph Greve3, Norbert Kockmann1, Thorsten Röder2

1 Department of Biochemical and Chemical Engineering, Equipment Design, TU Dortmund University, Emil-Figge-Str. 70, 44227 Dortmund/Germany 

2 Institute of Chemical Process Engineering, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Merck KGaA, Frankfurter Str. 250, 64293 Darmstadt/Germany    

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

New Derivatization Reagent for Detection of free Thiol-groups in Metabolites and Proteins in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Journal: ACS Publications, Analytical Chemistry

Annabelle Fülöp1, Tobias Bausbacher1, Stefano Rizzo2,Qiuqin Zhou1, Hartmut Gillandt2, Carsten Hopf1 and Miriam Rittner2

1Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany

2Sirius Fine Chemicals SiChem GmbH, Fahrenheitstr. 1, 28359 Bremen, Germany

Analysis of Calcium Signaling in Live Human Tongue Cell 3D-Cultures upon Tastant Perfusion

Journal: Cell Calcium, Volume 87, May 2020, 102164

 

Elena von Molitor a,*, Elina Nürnberg a,*, Torsten Ertongur-Fauth b, Paul Scholz b, Katja Riedel b, Mathias Hafner a, Rüdiger Rudolf a,c, Tiziana Cesetti a

a Institute of Molecular and Cell Biology, Hochschule Mannheim, 68163 Mannheim, Germany

b BRAIN AG, 64673 Zwingenberg, Germany

c Interdisciplinary Center for Neurosciences, Heidelberg University, 69120 Heidelberg, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Clinically Relevant OATP2B1 Inhibitors in Marketed Drug Space

Journal: Molecular Pharmaceutics

 

Unger MS.1,2,*, Mudunuru J.3, Schwab M.4,5, Hopf C.2, Drewes G.1, Nies AT.4, Zamek-Gliszczynski MJ.3, Reinhard FBM.1

 

1Cellzome, a GlaxoSmithKline Company , 69117 Heidelberg , Germany.

 

2Center for Mass Spectrometry and Optical Spectroscopy (CeMOS) and Institute of Medical Technology , Heidelberg University and Mannheim University of Applied Sciences , 68163 Mannheim , Germany

 

3Drug Metabolism and Disposition , GlaxoSmithKline , Collegeville , Pennsylvania 19426 , United States

 

4Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology , University of Tübingen , 70376 Stuttgart , Germany

 

5Departments of Clinical Pharmacology, Pharmacy and Biochemistry , University of Tübingen , 72074 Tübingen , Germany

 

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Jahr 2019

Model-based Scale-up Predictions: From Microto Millireactors using Inline FT-IR Spectroscopy

Journal: Organic Process Research & Development

 

Verena Fath1,2,*, Stefanie Szmais3, Philipp Lau3, Norbert Kockmann1 and Thorsten Röder2

 

1Department of Biochemical and Chemical Engineering, Equipment Design, TU Dortmund University, Emil-Figge-Str. 70, 44227 Dortmund, Germany 

 

2Institute of Chemical Process Engineering, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany 

 

3Merck KGaA, Frankfurter Str. 250, 64293 Darmstadt, Germany

 

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Mechanistic MALDI-TOF Cell-Based Assay for the Discovery of Potent and Specific Fatty Acid Synthase Inhibitors

Journal: Cell Chemical Biology, Volume 26, Issue 9

DavidWeigt1, Cynthia A.Parrish2, Julie A.Krueger3, Catherine A.Oleykowski3, Alan R.Rendina4, CarstenHopf1

 

1Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim Technical University, Paul-Wittsack-Strasse 10, 68163 Mannheim, Germany

 

2Medicinal Chemistry, GlaxoSmithKline, 1250 S Collegeville Road, Collegeville, PA 19426, USA

 

3Immuno-Oncology and Combinations Research Unit, GlaxoSmithKline, 1250 S Collegeville Road, Collegeville, PA 19426, USA

 

4Screening, Profiling and Mechanistic Biology, GlaxoSmithKline, 1250 S Collegeville Road, Collegeville, PA 19426, USA

Data fusion of GC-IMS data and FT-MIR spectra for the authentication of olive oils and honeys—is it worth to go the extra mile?

Journal: Analytical and Bioanalytical Chemistry, 1-15

 

Sebastian Schwolow1 & Natalie Gerhardt1 & Sascha Rohn2 & Philipp Weller1

 

1Institute for Instrumental Analytics and Bioanalysis, Mannheim University of Applied Sciences, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany 

 

2Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, 20146 Hamburg, Germany

The Influence of Different Fat Sources on Steatohepatitis and Fibrosis Development in the Western Diet Mouse Model of Non-alcoholic Steatohepatitis (NASH)

Journal: Original Research Article, Front. Physiol

 

Sebastian Schwolow1 & Natalie Gerhardt1 & Sascha Rohn2 & Philipp Weller1, Hannah K. Drescher1, Ralf Weiskirchen2,  Annabelle Fülöp3, Carsten Hopf3, Estibaliz González de San Román4, Pitter F. Huesgen4, Alain de Bruin5, Laura Bongiovanni5, Annette Christ6,7, René Tolba8, Christian Trautwein1 and Daniela C. Kroy1

 

1Department of Internal Medicine III, University Hospital RWTH Aachen, Aachen, Germany

2Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), University Hospital RWTH Aachen, Aachen, Germany

3Center for Biomedical Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Mannheim, Germany

4Central Institute for Engineering, Electronics and Analytics, ZEA-3 – Forschungszentrum Jülich, Jülich, Germany

5Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands

6Institute of Innate Immunity, University Hospital Bonn, Bonn, Germany

7Department of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA, United States

8Institute of Laboratory Animal Science and Experimental Surgery and Central Laboratory for Laboratory Animal Science, University Hospital RWTH Aachen, Aachen, Germany

In Situ Reaction Monitoring of Unstable Lithiated Intermediates through Inline FTIR Spectroscopy

Journal: Chemical Engineering Technology, 2019,42, No. 10, 1–11.

 

Verena Fath1,*, Prof. Dr. Norbert Kockmann2, Prof. Dr. Röder1

 

1Institute of Chemical Process Engineering, Mannheim University of Applied Sciences, Paul-Wittsack-Strasse 10, 68163 Mannheim, Germany.

 

2Department of Biochemical and Chemical Engineering, Equipment Design, TU Dortmund University, Emil-Figge-Strasse 70, 44227 Dortmund, Germany.

 

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Schnelle lokale Raman‐Messung zur Untersuchung von Konzentrationsprofilen bei Mischvorgängen in Mikrokanälen

Journal: Chemie Ingenieur Technik

 

Julian Deuerling1,*, Steffen Manser1,*, Julia Siber1,*, Shaun Keck1,*, Thomas M. Hufnagel1,*, Lukas Schmitt1,*, Isabel Medina1,*, Prof. Dr. Matthias Rädle1, Prof. Dr.-Ing. Jens-Uwe Repke2

 

1Hochschule Mannheim, CeMOS – Center for Mass Spectrometry and Optical Spectroscopy, Paul-Wittsack-Straße 10, 68163 Mannheim, Deutschland. 

 

2 Technische Universitaät Berlin, Fachgebiet Dynamik und Betrieb technischer Anlagen, Straße des 17. Juni 135, 10623 Berlin, Deutschland.

 

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

A customized multispectral needle probe combined with a virtual photometric setup for in vivo detection of Lewis lung carcinoma in an animal model. Measurement Science and Technology

Journal: Measurement Science and Technology, 2019

 

Frank Braun1,*, Robert Schalk1,*, Marcel Nachtmann1,*, Andreas Hien1,*, Rudolf Frank1,*, Thomas Beuermann2, Frank-Jürgen Methner3, Bettina Kränzlin4, Matthias Rädle1,5 and Norbert Gretz4,5 

 

1Center for Mass Spectrometry and Optical Spectroscopy, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany 

 

2Institute for Process Control, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany 

 

3Institute of Biotechnology, Technical University of Berlin, Chair of Brewing Science, Berlin, Germany 

 

4Medical Research Center, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany

 

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Lattice-Boltzmann Simulation and Experimental Validation of a Microfluidic T-Junction for Slug Flow Generation

Journal: Chemical Engineering, 2019, 3(2), 48

 

Volker Paul Schulz1, Nima Abbaspour1, Tobias Baumeister2 and Thorsten Röder2

 

1Department of Mechanical Engineering, Cooperative State University Mannheim, Coblitzallee 1-9, 68163 Mannheim

 

2Institute of Chemical Process Engineering, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany; t.baumeister@mail.de (T.B.)

A Novel Optical Tissue Clearing Protocol for Mouse Skeletal Muscle to Visualize Endplates in Their Tissue Context.

Journal: Frontiers in Cellular Neuroscience, 2019, 13, 49

 

Marion Patrick Ivey Williams1,*, Matteo Rigon1,*, Tatjana Straka1,2,*, Sarah Janice Hörner1,2,*, Manfred Thiel3, Norbert Gretz4,5, Mathias Hafner1,5, Markus Reischl6 and Rüdiger Rudolf1,2,5,7

 

1Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Mannheim, Germany

2Interdisciplinary Center for Neurosciences, Heidelberg University, Heidelberg, Germany

3Department of Anesthesiology and Surgical Intensive Care Medicine, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany

4Medical Faculty Mannheim, Medical Research Center, Heidelberg University, Mannheim, Germany

5Medical Faculty Mannheim, Institute of Medical Technology, Mannheim University of Applied Sciences, Mannheim, Germany

6Institute for Automation and Applied Informatics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany

7Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

 

Reactor Concept for Contactless Kinetic Measurement in Oscillating Droplets via Raman Spectroscopy

Journal: Chemie Ingenieur Technik, 2019, 91, No. 5, 1–7

Torsten Klement1,*, Prof. Dr. Thorsten Röder1, Prof. Dr.-Ing. Norbert Kockmann2

 

1Mannheim University of Applied Sciences, Institute of Chemical Process Engineering, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany. 

 

2TU Dortmund University, Faculty of Biochemical and Chemical Engineering, Laboratory of Equipment Design, Emil-FiggeStraße 70, 44227 Dortmund, Germany

 

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Bone Sialoprotein Shows Enhanced Expression in Early, High-Proliferation Stages of Three-Dimensional Spheroid Cell Cultures of Breast Cancer Cell Line MDA-MB-231.

Journal: Frontiers in Oncology, 2019, 9, 36

 

Valeh Rustamov1,2,*, Florian Keller1,2,*, Julia Klicks1,2,*, Mathias Hafner1,2 and Rüdiger Rudolf1,2

 

1Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Mannheim, Germany

2Institute of Medical Technology of Heidelberg University and Mannheim University of Applied Sciences, Mannheim, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Model-based scale-up and reactor design for solvent-free synthesis of an ionic liquid in a millistructured flow reactor

Journal: Reaction Chemistry & Engineering, 2019, 4, 523

 

Sebastian Schwolow1,*, Benedikt Mutsch1,*, Norbert Kockmann2 and  Thorsten Röder 

 

1Mannheim University of Applied Sciences, Institute of Chemical Process Engineering, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany

 

2TU Dortmund University, Biochemical and Chemical Engineering, Equipment Design, Emil-Figge-Straße 68, 44227 Dortmund, Germany

 

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Jahr 2018

Entwicklung eines Partikelmesssystems zur Erfassung geringer Streulicht‐Intensitäten optimiert für den Einsatz sehr schwacher Laserstrahlen

Chemie Ingenieur Technik

Teumer, T., Hufnagel, T., Schäfer, T., Schlarp‐Horvath, R., Karbstein, H.P., Methner, F.J. & Rädle, M.

Site-to-Site Reproducibility and Spatial Resolution in MALDI-MSI of Peptides from Formalin-Fixed Paraffin-Embedded Samples

Proteomics Clin. Appl. 2018, 1800029

Ly, A., Longuespée, R., Casadonte, R., Wandernoth, P., Schwamborn, K., Bollwein, C., Marsching, C., Kriegsmann, K., Hopf, C., Weichert, W., Kriegsmann, J., Schirmacher, P., Kriegsmann, M. & Deininger, S.-O.

A customized stand-alone photometric Raman sensor applicable in explosive atmospheres: a proof-of-concept study

Journal of Sensor and Sensor Systems

Nachtmann, M., Keck, S.P., Braun, F., Eckhardt, H.S., Mattolat, C., Gretz, N., Scholl, S. & Rädle, M.

Advanced MALDI mass spectrometry imaging in pharmaceutical research and drug development.

Curr. Opin. Biotechnol. 2019, 55, 51–59

Schulz, S., Becker, M., Groseclose, M. R., Schadt, S. & Hopf, C.

Automated analysis of lipid drug-response markers by combined fast and high-resolution whole cell MALDI mass spectrometry biotyping.

 Sci Rep. 2018 Jul 26; 8(1):11260.

Weigt, D., Sammour, DA., Ulrich, T., Munteanu, B. & Hopf, C.

Postnatal Development and Distribution of Sympathetic Innervation in Mouse Skeletal Muscle

International Journal of Molecular Sciences

Straka, T., Vita, V., Prokshi, K., Hörner, S.J., Khan, M.M., Pirazzini, M., Williams, M., Hafner, M., Zaglia, T. & Rudolf, R.

Deletion of Specific Sphingolipids in Distinct Neurons Improves Spatial Memory in a Mouse Model of Alzheimer’s Disease

Frontiers in Molecular Neuroscience
Front. Mol. Neurosci. 2018, 11, 206

Herzer, S., Hagan, C., von Gerichten, J., Dieterle, V., Munteanu, B., Sandhoff, R., Hopf, C., Nordström, V.

2017

In vitro skin three-dimensional models and their applications

Journal of Cellular Biotechnology,3: 21–39, 2017.

Klicks, J., von Molitor, E., Ertongur-Fauth, T., Rudolf, R. & Hafner, M.

Long-term 3D culture of the SCC4 cell line using three different culture methods and initial seeding densities.

Journal of Cellular Biotechnology, 3: 41–50, 2017;

Martí, Y., Nürnberg, E., Horschitz, S., Hafner, M., Schloss, P., Meyer-Lindenberg, A. & Lau, T.

Refinement of a neuronal differentiation protocol predominantly yields human iPS cell-derived dopaminergic neurons for the investigation of neurodegenerative pathomechanisms in vitro.

Journal of Cellular Biotechnology, vol. 3, no. 1, pp. 61-80, 2017

Martí, Y., Nürnberg, E., Horschitz, S., Hafner, M., Schloss, P., Meyer-Lindenberg, A. & Lau, T.

Resolution-optimized headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) for non-targeted olive oil profiling.

Analytical and Bioanalytical Chemistry
June 2017, Volume 409, Issue 16, pp 3933–3942

Gerhardt, N., Birkenmeier, M., Sanders, D., Rohn, S. & Weller, P.

Fourier Transform Infrared Microscopy Enables Guidance of Automated Mass Spectrometry Imaging to Predefined Tissue Morphologies

Sci Rep. 2018 Jan 10;8(1):313.

Rabe, J.-H.,  Sammour, D.A., Schulz, S., Munteanu, B., Ott, M., Ochs, K., Hohenberger, P., Marx, A., Platten, M., Opitz, C.A., Ory, D.S. & Hopf, C.