Jahr 2021

**Editor’s Pick 2021** - A Novel Optical Tissue Clearing Protocol for Mouse Skeletal Muscle to Visualize Endplates in Their Tissue Context

Journal: Frontiers in Cellular Neuroscience, doi.org/10.3389/fncel.2019.00049

M. P. I. Williams1,*, M. Rigon1,*, T. Straka1,2,*, S. J. Hörner1,2,*, M. Thiel3, N. Gretz4,5, M. Hafner1,5, M. Reischl6, R. Rudolf1,2,5,7

1Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Mannheim, Germany

2Interdisciplinary Center for Neurosciences, Heidelberg University, Heidelberg, Germany

3Department of Anesthesiology and Surgical Intensive Care Medicine, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany

4Medical Faculty Mannheim, Medical Research Center, Heidelberg University, Mannheim, Germany

5Medical Faculty Mannheim, Institute of Medical Technology, Mannheim University of Applied Sciences, Mannheim, Germany

6Institute for Automation and Applied Informatics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany

7Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

 

**Editor’s Pick 2021**

Die Veröffentlichung wurde vom Frontiers Editorial Office als Cellular Neuropathology Editor’s Pick 2021ausgewählt. Damit zeichnet die Redaktion “Artikel mit hervorragender wissenschaftlicher Qualität aus, die repräsentativ für die Arbeit in einem bestimmten Bereich sind".

Simultaneous self-optimisation of yield and purity through successive combination of inline FT-IR spectroscopy and online mass spectrometry in flow reactions

Journal: Journal of Flow Chemistry, doi.org/10.1007/s41981-021-00140-x

V. Fath1, P. Lau2, Ch. Greve2, P. Weller3, N. Kockmann4, T. Röder1

1Institute of Chemical Process Engineering, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163, Mannheim, Germany

2Merck KGaA, Frankfurter Str. 250, 64293, Darmstadt, Germany

3Institute of Instrumental Analytics and Bioanalysis, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163, Mannheim, Germany

3Department of Biochemical and Chemical Engineering, Equipment Design, TU Dortmund University, Emil-Figge-Str. 68, 44227, Dortmund, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Extracellular Matrix Components Regulate Bone Sialoprotein Expression in MDA-MB-231 Breast Cancer Cells

Journal: Cells, https://doi.org/10.3390/cells10061304

F. Keller1,2,*, R. Bruch1,*, F. Clauder3, M. Hafner1,2, R. Rudolf1,2

1Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, 68163 Mannheim, Germany

2Institute of Medical Technology, Heidelberg University and Mannheim University of Applied Sciences, 68167 Mannheim, Germany

3Immundiagnostik AG, 64625 Bensheim, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Oscillating droplet reactor – towards kinetic investigations in heterogeneous catalysis on a droplet scale

Journal: Reaction Chemestry & Engineering, doi: doi.org/10.1039/D0RE00466A

T. Klement1,*, Sch. Hanf2, F. Wolff1, N. Kockmann3, St. Schunk2, T. Röder1

1Mannheim University of Applied Sciences, Institute of Chemical, Process Engineering, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany

2hte GmbH, Kurpfalzring 104, 69123 Heidelberg, Germany

3Technische Universität Dortmund, Fakultät für Bio- und Chemieingenieurwesen, Apparatedesign, Emil-Figge-Straße 68, 44227 Dortmund, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany

Nitrogen monoxide as dopant for enhanced selectivity of isomeric monoterpenes in drift tube ion mobility spectrometry with 3 H ionization

Journal: Analytical and Bioanalytical Chemistry, doi: 10.1007/s00216-021-03306-7

R. Brendel1,*, S. Rohn2, P. Weller1

1Institute for Instrumental Analytics and Bioanalytics, Mannheim University of Applied Sciences, Paul-Wittsack-Strasse 10, 68163, Mannheim, Germany

2Hamburg School of Food Science, University of Hamburg, Grindelallee 117, 20146, Hamburg, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany

Preparation, Drug Treatment, and Immunohistological Analysis of Tri-Culture Spheroid 3D Melanoma-Like Model

Journal: Meladoma, doi.org/10.1007/978-1-0716-1205-7_13

M. Schäfer1,*, J. Klicks1,*, M. Hafner1, R. Rudolf1

1Institute of Molecular and Cell BiologyHochschule Mannheim, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Regulatory Function of Sympathetic Innervation on the Endo/Lysosomal Trafficking of Acetylcholine Receptor

Journal: Frontiers in Physiology, doi.org/10.3389/fphys.2021.626707

T. Straka1,2,3,*, Ch. Schröder4, A. Roos5,6, L. Kollipara4, A. Sickmann4,7,8, M. P. I. Williams1, M. Hafner1, M. M. Khan1,2,3*, R. Rudolf1,2,3*

1Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Mannheim, Germany

2Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe, Germany

3Interdisciplinary Center for Neurosciences, Heidelberg University, Heidelberg, Germany

4Leibniz-Institut für Analytische Wissenschaften—ISAS—e.V., Dortmund, Germany

5Department of Neuropediatrics, University Hospital Essen, Essen, Germany

6Children’s Hospital of Eastern Ontario Research Institute, Ottawa, ON, Canada

7Department of Chemistry, College of Physical Sciences, University of Aberdeen, Aberdeen, United Kingdom

8Medizinische Fakultät, Medizinische Proteom-Center (MPC), Ruhr-Universität Bochum, Bochum, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Kinetic Measurement of Acrylic Acid Polymerization at High Concentrations under Nearly Isothermal Conditions in a Pendula Slug Flow Reactor

Journal: Industrial & Engineering Chemistry Research, doi: 10.1021/acs.iecr.0c04732

T. Klement1, N. Kockmann2, Ch. Schwede3, T. Röder1

1Institute of Chemical Process Engineering, Mannheim University of Applied Sciences, Mannheim

2Faculty of Biochemical and Chemical Engineering, Laboratory of Equipment Design, TU Dortmund University, Dortmund

3BASF SE, RCP/ME-B01, Ludwigshafen am Rhein

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Fast Nanoliter-Scale Cell Assays Using Droplet Microarray–Mass Spectrometry Imaging

Journal: Advanced Biology, doi.org/10.1002/adbi.202000279

C. Ramallo Guevara1, D. Paulssen2, A. Popova2, C. Hopf1, P. Levkin2

1Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences Paul-Wittsack Str. 10, 68163 Mannheim, Germany

2Karlsruhe Institute of Technology (KIT) Institute of Biological and Chemical Systems – Functional Molecular, Systems (IBCS-FMS) Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany

Volatilomic Profiling of Citrus Juices by Dual-Detection HS-GC-MS-IMS and Machine Learning—An Alternative Authentication Approach

Journal: Journal of Agricultural and Food Chemistry, doi.org/10.1021/acs.jafc.0c07447

R. Brendel1,*, S. Schwolow2, S. Rohn2, P. Weller1

1Institute for Instrumental Analytics and Bioanalytics, Mannheim University of Applied Sciences, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany

2Institute of Food Chemistry, Hamburg School of Food Science, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Jahr 2020

Evaluation of semi-supervised learning using sparse labeling to segment cell nuclei

Journal: Current Directions in Biomedical Engineering, doi.org/10.1515/cdbme-2020-3103

R. Bruch1, R. Rudolf1, R. Mikut2, M. Reischl2

1Institute of Molecularand Cell Biology, Faculty of Biotechnology, Mannheim University ofApplied Sciences, Mannheim, Germany

2Institute for Automation and AppliedInformatics, Karlsruhe Institute of Technology, Karlsruhe, Germany

Recent developments of novel matrices and on-tissue chemical derivatization reagents for MALDI-MSI

Journal: Analytical and Bioanalytical Chemistry, doi: 10.1007/s00216-020-03023-7

Q. Zho1, A. Fülöp1, C. Hopf1

1Zentrum für Massenspektrometrie und optische Spektroskopie (CeMOS), Fachhochschule Mannheim, Paul-Wittsack-Str. 10, 68163, Mannheim, Deutschland

Comparision of PLSR, MCR-ALS and Kernel-PLSR for the quantification of allergenic fragrance compounds in complex cosmetic products based on nonlinear 2D GC-IMS data

Journal: Chemometrics and Intelligent Laboratory Systems, doi: 10.1016 / j.chemolab.2020.104128

R. Brendel1,*, S. Schwolow1, S. Rohn2, P. Weller1

1Institute for Instrumental Analytics and Bioanalytics, Mannheim University of Applied Sciences, 68163, Mannheim, Germany

2Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, 20146, Hamburg, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Computational Analysis of Alzheimer Amyloid Plaque Composition in 2D- and Elastically Reconstructed 3D-MALDI MS Images

Journal: Analytical Chemistry 2020, doi: 10.1021 / acs.analchem.0c02585

T. Enzlein1,*, J. Cordes1,*, B. Munteanu1, W. Michno2, L. Serneels3, B. De Strooper3, J. Hanrieder4, I. Wolf5, L. Chávez-Gutiérrez3, C. Hopf1

1Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Paul-Wittsack Str. 10, 68163 Mannheim, Germany

2Klinik für Psychiatrie und Neurochemie, Sahlgrenska-Akademie an der Universität Göteborg, Mölndal-Krankenhaus, Haus V3, Mölndal 43180, Schweden

3KU Leuven-VIB-Zentrum für Hirn- und Krankheitsforschung, VIB, Leuven 3000, Belgien ;  Klinik für Neurowissenschaften, Leuven Institut für Neurowissenschaften und Krankheiten, KU Leuven, Leuven 3000, Belgien ;  UK Dementia Research Institute an der UCL, University College London, London WC1E 6BT UK

4Klinik für Psychiatrie und Neurochemie, Sahlgrenska-Akademie der Universität Göteborg, Krankenhaus Mölndal, Haus V3, Mölndal 43180, Schweden ;  Abteilung für neurodegenerative Erkrankungen, University College London Queen Square Institut für Neurologie, Queen Square, London WC1N 3BG, Vereinigtes Königreich

5Fakultät für Informatik, Fachhochschule Mannheim, Paul-Wittsack-Straße 10, Mannheim 68163

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Spatially resolved mass spectrometry analysis of amyloid plaque-associated lipids

Journal: Journal of Neurochemistry, doi: 10.1111 / jnc.15216

M. Blank2, C. Hopf1

1Zentrum für Massenspektrometrie und optische Spektroskopie (CeMOS), Fachhochschule Mannheim, Paul-Wittsack-Str. 10, 68163, Mannheim, Deutschland.

2Center for Struktural Molecular (CEBIME/PROPESQ), Federal University of Santa Catarina, Florianopolis, Brazil.

An alternative pathway for sweet sensation: possible mechanisms and physiological relevance

Journal: European Journal of Physiology, doi:10.1007/s00424-020-02467-1

E. von Molitor1,*, K. Riedel2, M. Krohn2, R. Rudolf1,3, M. Hafner1, T. Cesetti1

1Institut für Molekular- und Zellbiologie, Hochschule Mannheim, 68163, Mannheim

2BRAIN AG, 64673, Zwingenberg, Deutschland

3Interdisziplinäres Zentrum für Neurowissenschaften, Universität Heidelberg, 69120, Heidelberg

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Feasibility Study for a Chemical Process Particle Size Characterization System for Explosive Environments Using Low Laser Power

Journal: Micromachines, doi:10.3390/mi11100911

J. Ross-Jones1,2, T. Teumer1,2, S. Wunsch1, L. Petri1, H. Nirschl2, M.J. Krause2, F.-J. Methner3, M. Rädle1

1Zentrum für Massenspektrometrie und optische Spektroskopie, Fachhochschule Mannheim, Paul-Wittsack-Straße 10, 68163 Mannheim.

2Forschungsgruppe Gitter Boltzmann, Institut für Maschinenbau, Verfahrenstechnik und Mechanik, Karlsruher Institut für Technologie, Straße am Forum 8, 76131 Karlsruhe.

3Lehrstuhl für Brauwissenschaft, Institut für Lebensmitteltechnologie und Lebensmittelchemie, Technische Universität Berlin, Seestraße 13, 13353 Berlin.

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Self-optimization of organic syntheses in an automated-flow microreactor system using Simplex and DoE

Journal: Chemie Ingenieur Technik, doi: 10.1002/cite.202055124

V. Fath1,*, P. Lau2, C. Greve2, N. Kockmann3

1Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

2Merck KGaA, Frankfurter Str. 250, 64293 Darmstadt/Germany  

3Department of Biochemical and Chemical Engineering, Equipment Design, TU Dortmund University, Emil-Figge-Str. 70, 44227 Dortmund, Germany 

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany

Kinetic measurements of acrylic acid polymerization in a pendular slug reactor

Journal: Chemie Ingenieur Technik, doi: 10.1002 / cite.202055217

T. Klement1,*, T. Röder1, N. Kockmann2

1Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

2Department of Biochemical and Chemical Engineering, Equipment Design, TU Dortmund University, Emil-Figge-Str. 70, 44227 Dortmund, Germany 

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

A Scaffold-Free 3-D Co-Culture Mimics the Major Features of the Reverse Warburg Effect In Vitro

Journal: Cells, doi: 0.3390/cells9081900

F. Keller1,2,*, R. Bruch, R. Schneider3, J. Meier-Hubberten3, M. Hafner1,2, R. Rudolf1,2

1Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, 68163 Mannheim, Germany

2Institute of Medical Technology, Medical Faculty Mannheim of Heidelberg University and Mannheim University of Applied Sciences, 68167 Mannheim, Germany

3TIP Oncology, Merck Healthcare KGaA, 64289 Darmstadt, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Gas-phase volatilomic approaches for quality control of brewing hops based on simultaneous GC-MS-IMS and machine learning

Journal: Analytical and Bioanalytical Chemistry, doi: 10.1007 / s00216-020-02842-y

R. Brendel1,*, S. Schwolow1, S. Rohn2, P. Weller1

1Paul-Wittsack-Straße 10, 68163, Mannheim

2Institut für Lebensmittelchemie, Hamburg School of Food Science, Universität Hamburg, Grindelallee 117, 20146, Hamburg

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Direct Automated MALDI Mass Spectrometry Analysis of Cellular Transporter Function: Inhibition of OATP2B1 Uptake by 294 Drugs.

Journal: Analytical Chemistry, https://doi.org/10.1021/acs.analchem.0c02186

M. S. Unger1,2,3,*, L. Schumacher1, T. Enzlein1, D. Weigt1, M.J. Zamek-Gliszczynski4, M. Schwab5,6,7,  A.T.  Nies5,6, G. Drewes3,  S. Schulz1,  F.B.M.  Reinhard3,  C. Hopf1,2,*

1Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Paul-Wittsack Str. 10, 68163 Mannheim, Germany

2Institute of Medical Technology, Heidelberg University and Mannheim University of Applied Sciences, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany

3Cellzome – a GlaxoSmithKline company, Meyerhofstr. 1, 69177 Heidelberg, Germany

4Drug  Metabolismand  Pharmacokinetics,  GlaxoSmithKline,  1250  S  Collegeville  Rd,  Collefeville, Pennsylvania 19426, United States

5Dr.  Margarete  Fischer-Bosch  Institute  for  Clinical  Pharmacology,  Auerbachstr.  112, 70376  Stuttgart, Germany

6iFit Cluster of Excellence (EXC2180) “Image Guided and Functionally Instructed Tumor Therapies”, University of Tübingen, Tübingen, Germany

7Departments   of   Clinical   Pharmacology,   Pharmacy   and   Biochemistry,   University   of   Tübingen, Geschwister Scholl Platz, 72074 Tübingen, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Recent Advances in Experimental Techniques for Flow and Mass Transfer Analyses in Thermal Separation Systems

Journal: Chemie Ingenieur Technik, doi: https://doi.org/10.1002/cite.202000076

U. Hampel1,2, M. Schubert1, A. Döß1, J. Sohr, V. Vishwakarma1, J.-U. Repke3, S.J. Gerke3, H. Leuner3, M. Rädle4, V. Kapoustina4, L. Schmitt4, M. Grünewald5, J.H. Brinkmann5, D. Plate5, E.Y. Kenig6, N. Lutters6, L. Bolenz6, F. Buckmann, D. Toye7, W. Arlt8, T. Linder8, R. Hoffmann9, H. Klein10, S. Rehfeldt10, T. Winkler10, H.-J. Bart11, D. Wirz11, J. Schulz11, S. Scholl12, W. Augustin12, K. Jasch12, F. Schlüter12, N. Schwerdtfeger12, S. Jahnke12, A. Jupke13, C. Kabatnik13, A.S. Braeuer14, M. D‘Auria14, T. Runowski15, M.F. Casal15, K. Becker15, A.-L. David15, A. Górak16, M. Skiborowski16, K. Groß16, H. Qammar16

1Helmholtz-Zentrum Dresden-Rossendorf, Institut für Fluiddynamik, Bautzner Landstraße 400, 01328 Dresden, Germany.

2Technische Universität Dresden, Institut für Energietechnik, 01062 Dresden, Germany.

3Technische Universität Berlin, Institut für Prozess- und Verfahrenstechnik, Straße des 17. Juni 135, 10623 Berlin, Germany.

4Hochschule Mannheim, Center for Mass Spectrometry and Optical Spectroscopy, John-Deere-Straße 81A, 68163 Mannheim, Germany.

5Ruhr-Universita¨t Bochum, Lehrstuhl für Fluidverfahrenstechnik, Universitätsstraße 150, 44801 Bochum, Germany.

6Universität Paderborn, Fakultat für Maschinenbau, Lehrstuhl für Fluidverfahrenstechnik, Pohlweg 55, 33098 Paderborn, Germany.

7Universite de Liege, Department of Chemical Engineering, Alle edu six Aout, 17B, 4000 Liege 1, Belgium.

8Friedrich-Alexander-Universität Erlangen-Nurnberg, Institute of Separation Science and Technology, Egerlandstraße 3, 91058 Erlangen, Germany.

9Linde Aktiengesellschaft, Engineering Division, Dr.-Carl-von-Linde-Straße 6–14, 82049 Pullach bei München, Germany.

10Technical University of Munich, Department of Mechanical Engineering, Institute of Plant and Process Technology, Boltzmannstraße 15, 85748 Garching, Germany.

11Technische Universität Kaiserslautern, Lehrstuhl für Thermische Verfahrenstechnik, PF 3049, 67653 Kaiserslautern, Germany.

12Technische Universität Braunschweig, Institute for Chemical and Thermal Process Engineering, Langer Kamp 7, 38106 Braunschweig, Germany.

13RWTH Aachen University, Aachener Verfahrenstechnik, Fluidverfahrenstechnik, Forckenbeckstraße 51, 52074 Aachen, Germany.

14Technische Universita¨t Bergakademie Freiberg (TUBAF), Institute of Thermal-, Environmental and Resources’ Process Engineering (ITUN), Leipziger Straße 28, 09599 Freiberg, Germany.

15Bayer AG, Engineering & Technology, Thermal Separation Technologies, 51368 Leverkusen, Germany.

16TU Dortmund University, Department of Biochemical and Chemical Engineering, Laboratory of Fluid Separations, Emil-Figge-Straße 70, 44227 Dortmund, Germany.

Fast Quantification Without Conventional Chromatography, the Growing Power of Mass Spectrometry

Journal: Analytical Chemistry, doi: 10.1021/acs.analchem.0c00877

G. Gachumi1, R.W. Purves1,2, C. Hopf3, A. El-Aneed1

1College of Pharmacy and Nutrition, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK, Canada, S7N 5E5.

2Centre for Veterinary Drug Residues, Canadian Food Inspection Agency, 116 Veterinary Rd, Saskatoon, SK, Canada, S7N 2R3.

3Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Paul-Wittsack-Strasse 10, 68163 Mannheim, Germany.

Lab‐scale microreactor plant for the study of methylations with liquid chloromethane

Journal: Chemical Engineering Technology, doi.org/10.1002/ceat.202000011

M. Sc. C.V. Benzin1,2,*,Prof. Dr.-Ing. N. Kockmann1, Prof. Dr. T. Röder2

1TU Dortmund University, Laboratory of Equipment Design, Emil-Figge-Straße 68, 44227 Dortmund, Germany

2Hochschule Mannheim University of Applied Sciences, Institute of Chemical Process Engineering, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany

Self-optimising processes and real-timeoptimisation of organic syntheses in a microreactor system using Nelder–Mead and design of experiments

Journal: Reaction Chemistry & Engineering, doi: 10.1039/d0re00081g

V. Fath3,*, N. Kockmann1, J. Otto2, T. Röder3 

1Department of Biochemical and Chemical Engineering, Equipment Design, TU Dortmund University, Emil-Figge-Str. 70, 44227 Dortmund, Germany

2Institute of Chemical Process Engineering, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany

3Hochschule Mannheim University of Applied Sciences, Institute of Chemical Process Engineering, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany 

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany

Efficient Kinetic Data Acquisition and Model Prediction: Continuous Flow Microreactors, Inline FT-IR Spectroscopy, and Self-modeling Curve Resolution

Journal: Organic Process Research & Development, doi.org/10.1021/acs.oprd.0c00037

V. Fath1,2, *, P. Lau3, Chr. Greve3, N. Kockmann1, T. Röder2

1Department of Biochemical and Chemical Engineering, Equipment Design, TU Dortmund University, Emil-Figge-Str. 70, 44227 Dortmund/Germany 

2Institute of Chemical Process Engineering, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

3Merck KGaA, Frankfurter Str. 250, 64293 Darmstadt/Germany    

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

New Derivatization Reagent for Detection of free Thiol-groups in Metabolites and Proteins in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Journal: Analytical Chemistry, doi.org/10.1021/acs.analchem.9b05630

A. Fülöp1, T. Bausbacher1, St. Rizzo2, Q. Zhou1, H. Gillandt2, C. Hopf1, M. Rittner2

1Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, 68163 Mannheim, Germany

2Sirius Fine Chemicals SiChem GmbH, 28359 Bremen, Germany

Molecule Sensitive Optical Imaging and Monitoring Techniques-A Review of Applications in Micro-Process Engineering.

Journal: Chemie Ingenieur Technik, doi:10.1002/cite.202000076

M. Nachtmann1, J. Deuerling1,*, M. Rädle1

1Forschungszentrum CeMOS, Fachhochschule Mannheim, Paul-Wittsack-Str. 10, 68163 Mannheim, Deutschland

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany

Sensing Senses: Optical Biosensors to Study Gustation

Journal: Sensors (Basel), doi: https://doi.org/10.3390/s20071811

E. von Molitor1,*, K. Riedel2, M. Hafner1, R.Rudolf1,3, T. Cesetti1

1Institut für Molekular- und Zellbiologie, Hochschule Mannheim, 68163 Mannheim

2BRAIN AG, 64673 Zwingenberg

3Interdisziplinäres Zentrum für Neurowissenschaften, Universität Heidelberg, 69120 Heidelberg

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Routine Optical Clearing of 3D-Cell Cultures: Simplicity Forward

Journal: Frontiers in Molecular Biosiences, doi.org/10.3389/fmolb.2020.00020

E. Nürnberg1,2,*, M. Vitacolonna1, J. Klicks1,*, E. von Molitor1,*, T. Cesetti1, F. Keller1,*, R. Bruch1,*, T. Ertongur-Fauth3, K. Riedel3, P. Scholz3, T. Lau2, R. Schneider4, J. Meier4, M. Hafner1, R. Rudolf1

1Institut für Molekular- und Zellbiologie, Fakultät für Biotechnologie, Fachhochschule Mannheim, Mannheim

2Zentralinstitut für Seelische Gesundheit, Abteilung für translationale Hirnforschung, Medizinische Fakultät Mannheim, Universität Heidelberg, Mannheim

3BRAIN AG, Zwingenberg

4TIPP Onkologie, Merck Healthcare KGaA, Darmstadt

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

A paper-based colorimetric sensor array for discrimination of monofloral European honeys based on gold nanoparticles and chemometrics data analysis

Journal: Molecular and Biomolecular Spectroscopy, https://doi.org/10.1016/j.saa.2020.119076

M. Chaharlangi1, J. Tashkhourian1, M. mahdi Bordbar1R. Brendel2,*, P. Weller2, B. Hemmateenejad1,3

1Abteilung für Chemie, Universität Shiraz, Shiraz 71454, Iran

2Institut für Instrumentelle Analytik und Bioanalytik, Fachhochschule Mannheim, 68163 Mannheim

3Institut für Medizinische und Pharmazeutische Chemie, Technische Universität Braunschweig, Braunschweig

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Analysis of Calcium Signaling in Live Human Tongue Cell 3D-Cultures upon Tastant Perfusion

Journal: Cell Calcium, doi.org/10.1016/j.ceca.2020.102164

E. von Molitor 1,*, E. Nürnberg 1,*, T. Ertongur-Fauth 2, P. Scholz 2, K. Riedel 2, M. Hafner 1, R. Rudolf 1,3, T. Cesetti 1

1Institute of Molecular and Cell Biology, Hochschule Mannheim, 68163 Mannheim, Germany

BRAIN AG, 64673 Zwingenberg, Germany

3Interdisciplinary Center for Neurosciences, Heidelberg University, 69120 Heidelberg, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Jahr 2019

Towards optimized breast cancer 3D spheroid mono- and co-culture models for pharmacological research and screening

Journal of Cellular Biotechnolog, doi: 10.3233/JCB-199001

F. Keller1,*, R. Rudolf1,2, M. Hafner1,2

1Institute of Molecular and Cellular Biology, Mannheim University of Applied Sciences, Mannheim, Germany.

2Institute of Medical Technology of Heidelberg University and Mannheim University of Applied Sciences, Mannheim, Germany.

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany.

Clinically Relevant OATP2B1 Inhibitors in Marketed Drug Space

Journal: Molecular Pharmaceuticsdoi.org/10.1021/acs.molpharmaceut.9b00897

M.S. Unger1,*, Matthias Schwab1,4,5, M.J. Zamek-Gliszczynski1, G. Drewes2, F.B.M. Reinhard2, C. Hopf3, J. Mudunuru3,4, A.T. Nies4

1Drug Metabolism and Disposition, GlaxoSmithKline, Collegeville, Pennsylvania 19426, United States.

2Cellzome, a GlaxoSmithKline Company, 69117 Heidelberg, Germany.

3Center for Mass Spectrometry and Optical Spectroscopy (CeMOS) and Institute of Medical Technology, Heidelberg University and Mannheim University of Applied Sciences, 68163 Mannheim, Germany.

4Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, University of Tübingen, 70376 Stuttgart, Germany.

5Departments of Clinical Pharmacology, Pharmacy and Biochemistry, University of Tübingen, 72074 Tübingen, Germany.

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Contrast enhancement of surface layers with fast middle-infrared scanning

Journal: Heliyon, doi: 10.1016/j.heliyon.2019.e02442

T. Kümmel1,3, T. Teumer1,2, P. Dörnhofer1, F.-J. Methner2, B. Wängler3, M. Rädle1

1Center for Mass Spectrometry and Optical Spectroscopy, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, Mannheim, 68163, Germany

2Institute of Food Technology and Food Chemistry, Technische Universität Berlin, Seestraße 13, Berlin, 13353, Germany

3University of Heidelberg, Medical Faculty Mannheim, Theodor-Kutzer-Ufer 1-3, Mannheim, 68167, Germany

Model-based Scale-up Predictions: From Microto Millireactors using Inline FT-IR Spectroscopy

Journal: Organic Process Research & Development, doi.org/10.1021/acs.oprd.9b00265

V. Fath1,2,*, St. Szmais3, P. Lau3, N. Kockmann1, T. Röder2

1Department of Biochemical and Chemical Engineering, Equipment Design, TU Dortmund University, Emil-Figge-Str. 70, 44227 Dortmund, Germany

2Institute of Chemical Process Engineering, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany

3Merck KGaA, Frankfurter Str. 250, 64293 Darmstadt, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Investigation of local layer thickness distributions in fluids with the help of near-infrared image analysis

Journal: Chemie Ingenieur Technik, doi: 10.1002/cite.201800106

I. Medina1, L. Schmitt1, V. Kapoustina1, M. Rädle1, St. Scholl2

1Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences Paul-Wittsack Str. 10, 68163 Mannheim, Germany

2Technische Universita¨t Braunschweig, Institut für Chemische und Thermische Verfahrenstechnik, Langer Kamp 7, 38106 Braunschweig, Germany

Mechanistic MALDI-TOF Cell-Based Assay for the Discovery of Potent and Specific Fatty Acid Synthase Inhibitors

Journal: Cell Chemical Biology, doi.org/10.1016/j.chembiol.2019.06.004

D. Weigt1,*, C. A.Parrish2, J. A.Krueger3, C. A.Oleykowski3, Al. R.Rendina4, C. Hopf1

1Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim Technical University, Paul-Wittsack-Strasse 10, 68163 Mannheim, Germany

2Medicinal Chemistry, GlaxoSmithKline, 1250 S Collegeville Road, Collegeville, PA 19426, USA

3Immuno-Oncology and Combinations Research Unit, GlaxoSmithKline, 1250 S Collegeville Road, Collegeville, PA 19426, USA

4Screening, Profiling and Mechanistic Biology, GlaxoSmithKline, 1250 S Collegeville Road, Collegeville, PA 19426, USA

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Data fusion of GC-IMS data and FT-MIR spectra for the authentication of olive oils and honeys—is it worth to go the extra mile?

Journal: Analytical and Bioanalytical Chemistry, doi: 10.1007/s00216-019-01978-w

S. Schwolow1, N. Gerhardt1, S. Rohn2, P. Weller1

1Institute for Instrumental Analytics and Bioanalysis, Mannheim University of Applied Sciences, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany

2Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, 20146 Hamburg, Germany

The Influence of Different Fat Sources on Steatohepatitis and Fibrosis Development in the Western Diet Mouse Model of Non-alcoholic Steatohepatitis (NASH)

Journal: Frotiers in Physiology, doi.org/10.3389/fphys.2019.00770

S. Schwolow1, N. Gerhardt1, S. Rohn2, P. Weller1, H. K. Drescher1, R. Weiskirchen2,  A. Fülöp3, C. Hopf3, E. González de San Román4, P. F. Huesgen4, A. de Bruin5, L. Bongiovanni5, A. Christ6,7, R. Tolba8, Ch. Trautwein1, D. C. Kroy1

1Department of Internal Medicine III, University Hospital RWTH Aachen, Aachen, Germany

2Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), University Hospital RWTH Aachen, Aachen, Germany

3Center for Biomedical Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Mannheim, Germany

4Central Institute for Engineering, Electronics and Analytics, ZEA-3 – Forschungszentrum Jülich, Jülich, Germany

5Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands

6Institute of Innate Immunity, University Hospital Bonn, Bonn, Germany

7Department of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA, United States

8Institute of Laboratory Animal Science and Experimental Surgery and Central Laboratory for Laboratory Animal Science, University Hospital RWTH Aachen, Aachen, Germany

In Situ Reaction Monitoring of Unstable Lithiated Intermediates through Inline FTIR Spectroscopy

Journal: Chemical Engineering Technology, doi.org/10.1002/ceat.201900074

Verena Fath1,*, Prof. Dr. Norbert Kockmann2, Prof. Dr. Röder1

1Institute of Chemical Process Engineering, Mannheim University of Applied Sciences, Paul-Wittsack-Strasse 10, 68163 Mannheim, Germany.

2Department of Biochemical and Chemical Engineering, Equipment Design, TU Dortmund University, Emil-Figge-Strasse 70, 44227 Dortmund, Germany.

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Fast local Raman measurement to investigate concentration profiles during mixing processes in microchannels

Journal: Chemie Ingenieur Technik, doi.org/10.1002/cite.201800105

J. Deuerling1,*, St. Manser1,*, J. Siber1,*, Sh. Keck1,*, Th. M. Hufnagel1,*, L. Schmitt1,*, I. Medina1,*, Prof. Dr. Matthias Rädle1, Prof. Dr.-Ing. Jens-Uwe Repke2

1Hochschule Mannheim, CeMOS – Center for Mass Spectrometry and Optical Spectroscopy, Paul-Wittsack-Straße 10, 68163 Mannheim, Deutschland.

2 Technische Universitaät Berlin, Fachgebiet Dynamik und Betrieb technischer Anlagen, Straße des 17. Juni 135, 10623 Berlin, Deutschland.

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

A customized multispectral needle probe combined with a virtual photometric setup for in vivo detection of Lewis lung carcinoma in an animal model. Measurement Science and Technology

Journal: Measurement Science and Technology, doi.org/10.1088/1361-6501/ab24a1

F. Braun1,*, R. Schalk1,*, M. Nachtmann1,*, A. Hien1,*, R. Frank1,*, Th. Beuermann2, F.-J. Methner3, B. Kränzlin4, M. Rädle1,5, N. Gretz4,5

1Center for Mass Spectrometry and Optical Spectroscopy, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany

2Institute for Process Control, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany

3Institute of Biotechnology, Technical University of Berlin, Chair of Brewing Science, Berlin, Germany

4Medical Research Center, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Lattice-Boltzmann Simulation and Experimental Validation of a Microfluidic T-Junction for Slug Flow Generation

Journal: Chemical Engineering, doi.org/10.3390/chemengineering3020048

V. Paul Schulz1, N. Abbaspour1, T. Baumeister2 , T. Röder2

1Department of Mechanical Engineering, Cooperative State University Mannheim, Coblitzallee 1-9, 68163 Mannheim

2Institute of Chemical Process Engineering, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany; t.baumeister@mail.de (T.B.)

A novel spheroid-based co-culture model mimics loss of keratinocyte differentiation, melanoma cell invasion, and drug-induced selection of ABCB5-expressing cells

Journal: BMC Cancer, doi: 10.1186/s12885-019-5606-4

J. Klicks1,2,*, C. Maßlo3, A. Kluth4, R. Rudolf1,2, M. Hafner1,2

1Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany.

2Institute of Medical Technology, Mannheim University of Applied Sciences and Medical Faculty Mannheim of Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany. 

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany

 

Quality assessment of olive oils based on temperature-ramped HS-GC-IMS and sensory evaluation: Comparison of different processing approaches by LDA, kNN, and SVM

Journal: Food Chemistry, doi: 10.1016/j.foodchem.2018.11.095

N. Gerhardt1, S. Schwolow1, S. Rohn2, P. RuizPérez-Cacho3, H. Galán-Soldevilla3, L. Arced4, P. Weller1

1Institute for Instrumental Analytics and Bioanalysis, Mannheim University of Applied Sciences, 68163 Mannheim, Germany

2Institute of Food Chemistry, Hamburg School of Food Science, University of Hamburg, 20146 Hamburg, Germany

3Laboratorio de Estudios Sensoriales (GrupoSens), Departamento de Bromatología y Tecnología de los Alimentos, Universidad de Córdoba. Campus de Rabanales, 14070 Córdoba, Spain

4Department of Analytical Chemistry, Institute of Fine Chemistry and Nanochemistry, University of Córdoba, Campus de Rabanales, Marie Curie Annex Building, 14071 Córdoba, Spain

Influence of a single microstructure on local mass transfer in liquid film flows

Journal: Chemical Engineering Research and Design, doi: 10.1016/j.cherd.2019.04.022

V. Kapoustina1, J. Guffart1, A. Hien1, J. Strischakov1, I. Medina1, M. Rädle1, J. Repke2

1Center for Mass Spectrometry and Optical Spectroscopy, Mannheim University of Applied Sciences, Mannheim, Germany.

2Chair of Process Dynamics and Operation, Technische Universität Berlin, Berlin, Germany.

A Novel Optical Tissue Clearing Protocol for Mouse Skeletal Muscle to Visualize Endplates in Their Tissue Context.

Journal: Frontiers in Cellular Neuroscience, doi.org/10.3389/fncel.2019.00049

M. P. I. Williams1,*, M. Rigon1,*, T. Straka1,2,*, S. J. Hörner1,2,*, M. Thiel3, N. Gretz4,5, M. Hafner1,5, M. Reischl6, R. Rudolf1,2,5,7

1Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Mannheim, Germany

2Interdisciplinary Center for Neurosciences, Heidelberg University, Heidelberg, Germany

3Department of Anesthesiology and Surgical Intensive Care Medicine, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany

4Medical Faculty Mannheim, Medical Research Center, Heidelberg University, Mannheim, Germany

5Medical Faculty Mannheim, Institute of Medical Technology, Mannheim University of Applied Sciences, Mannheim, Germany

6Institute for Automation and Applied Informatics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany

7Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

 

 

Reactor Concept for Contactless Kinetic Measurement in Oscillating Droplets via Raman Spectroscopy

Journal: Chemie Ingenieur Technik, doi.org/10.1002/cite.201800199

T. Klement1,*, Prof. Dr. T. Röder1, Prof. Dr.-Ing. N. Kockmann2

1Mannheim University of Applied Sciences, Institute of Chemical Process Engineering, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany.

2TU Dortmund University, Faculty of Biochemical and Chemical Engineering, Laboratory of Equipment Design, Emil-FiggeStraße 70, 44227 Dortmund, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Bone Sialoprotein Shows Enhanced Expression in Early, High-Proliferation Stages of Three-Dimensional Spheroid Cell Cultures of Breast Cancer Cell Line MDA-MB-231.

Journal: Frontiers in Oncology, doi.org/10.3389/fonc.2019.00036

V. Rustamov1,2,*, F. Keller1,2,*, J. Klicks1,2,*, M. Hafner1,2, R. Rudolf1,2

1Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Mannheim, Germany

2Institute of Medical Technology of Heidelberg University and Mannheim University of Applied Sciences, Mannheim, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Advanced MALDI mass spectrometry imaging in pharmaceutical research and drug development

Journal: Current Opinion in Biotechnolgy, doi: 10.1016/j.copbio.2018.08.003

S. Schulz1, M. Becker2, M.R. Groseclose3, S. Schadt4, C. Hopf1

1Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Mannheim, Germany.

2Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach/Riss, Germany.

3Bioimaging US, GlaxoSmithKline, King of Prussia, PA 19406, United States.

4Roche Pharmaceutical Research and Early Development, Pharmaceutical Sciences, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd., CH-4070 Basel, Switzerland.

Model-based scale-up and reactor design for solvent-free synthesis of an ionic liquid in a millistructured flow reactor

Journal: Reaction Chemistry & Engineering, doi: 10.1039/c8re00148k

S. Schwolow1,*, B. Mutsch1,*, N. Kockmann2, T. Röder 

1Mannheim University of Applied Sciences, Institute of Chemical Process Engineering, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany

2TU Dortmund University, Biochemical and Chemical Engineering, Equipment Design, Emil-Figge-Straße 68, 44227 Dortmund, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

A cationic near infrared fluorescent agent and ethyl-cinnamate tissue clearing protocol for vascular staining and imaging

Journal: Scientific Reports, doi: 10.1038/s41598-018-36741-1

J. Huang1,2, C. Brenna1,2, A.U. Khan1,2, C. Daniele1,2, R. Rudolf2,3, V. Heuveline4, N. Gretz1,2

1Medical Research Center, Medical Faculty Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.

2Institute for Medical Technology, University of Heidelberg and University of Applied Sciences, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.

3Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, 68163, Mannheim, Germany.

4Director of the Computing Centre, Heidelberg University, Im Neuenheimer Feld 293, 69120, Heidelberg, Germany. 

Jahr 2018

Development of a particle measuring system for recording the scattered light intensities, optimized for the use of very weak laser beams

Journal: Chemie Ingenieur Technik, doi: https://doi.org/10.1002/cite.201800073

T. Teumer1, T. Hufnagel1,,*, T. Schäfer1, R. Schlarp‐Horvath1, H.P. Karbstein2, F.J. Methner3, M. Rädle1

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

1Hochschule Mannheim, CeMOS – Center of mass spectrometry and optical spectroscopy, Paul-Wittsack-Straße 10, 68163 Mannheim, Deutschland.

2KIT, Institut für Bio- und Lebensmitteltechnik, Teilinstitut I: Lebensmittelverfahrenstechnik, Kaiserstraße 12, 76131 Karlsruhe, Deutschland.

3Technische Universita¨t Berlin, Institut für Lebensmitteltechnologie und Lebensmittelchemie, Fachgebiet Brauwesen, Straße des 17. Juni 135, 10623 Berlin, Deutschland.

Site-to-Site Reproducibility and Spatial Resolution in MALDI-MSI of Peptides from Formalin-Fixed Paraffin-Embedded Samples

Journal: Proteomics Clinical Applications, doi: https://doi.org/10.1002/prca.201800180

A. Ly5, R. Longuespée6, R. Casadonte1, P. Wandernoth1, K. Schwamborn2, Ch. Bollwein2, Ch. Marsching3, K. Kriegsmann4, C. Hopf3, W. Weichert2, J. Kiregsmann1, P. Schirmacher6, M. Kriegsmann6, S.-O. Deininger5

1Proteopath GmbH, Trier, Germany. 

2Institute of Pathology Technical University of Munich Munich, Germany. 

3Center for Biomedical Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Mannheim, Germany.

4Department of Hematology Oncology and Rheumatology, University Hospital Heidelberg, Heidelberg, Germany. 

5Bruker Daltonik GmbH, Bremen, Germany.

6Institute of Pathology, University Hospital Heidelberg, Heidelberg, Germany

A customized stand-alone photometric Raman sensor applicable in explosive atmospheres: a proof-of-concept study

Journal: Journal of Sensor and Sensor Systems, doi.org/10.5194/jsss-7-543-2018

M. Nachtmann1S.P. Keck1,*, F. Braun1, H. S. Eckhardt2, Chr. Mattolat2, N. Gretz3, St. Scholl4, M. Rädle1

1Institute for Process Control and Innovative Energy Conversion, Mannheim University of Applied Sciences, Mannheim, 68163, Germany.

2tec5 AG, Oberursel/Ts, 61440, Germany.

3Medical Research Center, Medical Faculty Mannheim, Heidelberg University, Mannheim, 68167, Germany.

4Institute for Chemical and Thermal Process Engineering, Technical University Braunschweig, Braunschweig, 38106, Germany.

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Advanced MALDI mass spectrometry imaging in pharmaceutical research and drug development.

Journal: Current Opinion in Biotechnology, doi: https://doi.org/10.1016/j.copbio.2018.08.003

S. Schulz1, M. Becker2, M. Reid Groseclose3, S. Schadt4, C. Hopf1

1Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Mannheim, Germany

2Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach/Riss, Germany

3Bioimaging US, GlaxoSmithKline, King of Prussia, PA 19406, United States

4Roche Pharmaceutical Research and Early Development, Pharmaceutical Sciences, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd., CH-4070 Basel, Switzerland

Automated analysis of lipid drug-response markers by combined fast and high-resolution whole cell MALDI mass spectrometry biotyping.

Journal: Scienetific Reports, doi.org/10.1038/s41598-018-29677-z

D. Weigt1,2, D. A. Sammour1, T. Ulrich1, B. Munteanu1, C. Hopf1,2

1Center for biomedical Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Paul-Wittsack Str. 10, 68163, Mannheim, Germany.

2HBIGS International Graduate School of Molecular and Cellular Biology, Heidelberg University, Im Neuenheimer Feld 501, 69120, Heidelberg, Germany. 

Postnatal Development and Distribution of Sympathetic Innervation in Mouse Skeletal Muscle

Journal: International Journal of Molecular Sciences, doi: 10.3390/ijms19071935

T. Straka1,2,3, V. Vita4, K. Prokshi1,2S. J. Hörner1,2,*, M.M. Khan1,2,3, M. Pirazzini4, M.P.I. Williams1,M. Hafner1, T. Zaglia4,5,6, R. Rudolf1,2,3,7

1Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, 68163 Mannheim, Germany.

2Interdisciplinary Center for Neurosciences, Heidelberg University, 69120 Heidelberg, Germany.

3Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, 76344 Eggenstein-Leopoldshafen, Germany.

4Venetian Institute of Molecular Medicine, 35129 Padua, Italy.

5Department of Biomedical Sciences, University of Padua, 35131 Padua, Italy.

6Department of Cardiac Thoracic and Vascular Sciences, University of Padua, 35128 Padua, Italy.

7Author to whom correspondence should be addressed.

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Deletion of Specific Sphingolipids in Distinct Neurons Improves Spatial Memory in a Mouse Model of Alzheimer’s Disease

Journal: Frontiers in Molecular Neuroscience, doi: https://doi.org/10.3389/fnmol.2018.00206

S. Herzer1,2, C. Hagan1,3, J. von Gerichten1,4, V. Dieterle1,2, B. Munteanu5, R. Sandhoff1,4, C. Hopf5, V. Nordström1,2*

1Division of Cellular and Molecular Pathology, German Cancer Research Center, Heidelberg, Germany.

2Interdisciplinary Center for Neurosciences, Heidelberg University, Heidelberg, Germany.

3Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, United States.

4Lipid Pathobiochemistry Group, German Cancer Research Center, Heidelberg, Germany.

5Center for Mass Spectrometry (CeMOS), University of Heidelberg and Mannheim University of Applied Sciences, Mannheim, Germany.

Volatile-Compound Fingerprinting by Headspace-Gas-Chromatography Ion-Mobility Spectrometry (HS-GC-IMS) as a Benchtop Alternative to 1H NMR Profiling for Assessment of the Authenticity of Honey.

Journal: Analytical Chemistry, doi.org/10.1021/acs.analchem.7b03748

N. Gerhardt1, M. Birkenmeier1, S. Schwolow2, S. Rohn1, P. Weller1

1Institute for Instrumental Analytics and Bioanalysis, Mannheim University of Applied Sciences, 68163 Mannheim, Germany.

2Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, 20146 Hamburg, Germany.

2017

In vitro skin three-dimensional models and their applications

Journal: Journal of Cellular Biotechnology, doi: 10.3233/JCB-179004

J. Klicks1,*, E. von Molitor1,*, T. Ertongur-Fauth2, R. Rudolf 1,3, M. Hafner1,3

1Mannheim University of Applied Sciences, Institute of Molecular and Cell Biology, Mannheim, Germany.

2BRAIN AG, Zwingenberg, Germany c Institute of Medical Technology, Heidelberg University, Heidelberg, Germany.

3Institute of Medical Technology, Heidelberg University, Heidelberg, Germany.

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Long-term 3D culture of the SCC4 cell line using three different culture methods and initial seeding densities.

Journal of Cellular Biotechnology, doi: 10.3233/JCB-179005

V. Rustamov1,2, R. Rudolf1,2, V. Yagublu3, H.-M.Kuhn1, M. Vitacolonna1, M. Hafner1,2

1Institute of Molecular and Cell Biology, Faculty of Biotechnology, Mannheim University of Applied Sciences, Mannheim, Germany.

2Institute of Medical Technology, Heidelberg University, Heidelberg, Germany.

2Department of General, Visceral, Vascular, and Thoracic Surgery, Frankfurt Hoechst Hospital, Frankfurt, Germany.

Refinement of a neuronal differentiation protocol predominantly yields human iPS cell-derived dopaminergic neurons for the investigation of neurodegenerative pathomechanisms in vitro.

Journal: Journal of Cellular Biotechnology, doi:10.3233/JCB-179007

Y, Martí2,1, E. Nürnberg3,1,*, S. Horschitz2, M. Hafner3, P. Schloss2, AMeyer-Lindenberg2, T. Lau1

1Central Institute of Mental Health, Hector Institute for Translational Brain Research, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.

2Central Institute of Mental Health, Medical Faculty Mannheim, Heidelberg University, Department Psychiatry and Psychotherapy, Mannheim, Germany.

 3Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Mannheim, Germany.

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Resolution-optimized headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) for non-targeted olive oil profiling.

Journal: Analytical and Bioanalytical Chemistry, doi.org/10.1007/s00216-017-0338-2

N. Gerhardt1, M. Birkenmeier1, D. Sanders2, S. Rohn3, P. Weller1

1Institute for Instrumental Analytics and Bioanalysis, Mannheim University of Applied Sciences, Paul-Wittsack-Strasse 10, 68163 Mannheim, Germany.

2Gesellschaft für Analytische Sensorsysteme mbH, Otto-Hahn-Straße 15, 44227 Dortmund, Germany 3 Hamburg School of Food Science, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany.

Fourier Transform Infrared Microscopy Enables Guidance of Automated Mass Spectrometry Imaging to Predefined Tissue Morphologies

Journal: Scientific Reports, doi: 10.1038/s41598-017-18477-6. 

J.-H. Rabe1,2, D.A. Sammour1,2, S. Schulz1,2, B. Munteanu1,2, M. Ott3, K. Ochs3,6, P. Hohenberger4, A. Marx4, M. Platten3,4, Chr.A. Opitz5,6, D.S. Ory7, C. Hopf1,2

1Center for Applied Research in Applied Biomedical Mass Spectrometry (ABIMAS), Mannheim University of Applied Sciences, Paul-Wittsack Str. 10, 68163, Mannheim, Germany.

2Institute of Medical Technology, Heidelberg University and Mannheim University of Applied Sciences, Paul-Wittsack Str. 10, 68163, Mannheim, Germany.

3German Cancer Consortium (DKTK) CCU Neuroimmunology and Brain Tumor Immunology, German Cancer Research Center (DKFZ), Heidelberg, Germany.

4University Medical Center Mannheim of Heidelberg University, Mannheim, Germany.

5Brain Cancer Metabolism Group, German Cancer Research Center (DKFZ), Heidelberg, Germany.

6Department of Neurology and National Center of Tumor Diseases, University Hospital Heidelberg, Heidelberg, Germany.

7Diabetic Cardiovascular Disease Center and Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, 63110, USA. Correspondence.

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany.

Hochschule Mannheim
M²Aind Projektkoordination
Paul-Wittsack-Strasse 10
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+49 621 292-6579
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