Year 2021

Label-free cell assays to determine compound uptake or drug action using MALDI-TOF mass spectrometry

Journal: Nature Protocols (2021)

Melissa Unger1, Martina Blank1, Thomas Enzlein1, Carsten Hopf1

1Faculty of Computer Science, Mannheim University of Applied Sciences, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany.

Tryptophan metabolism is inversely regulated in the tumor and blood of patients with glioblastoma

Journal: Theranostics, doi:10.7150/thno.60679

Verena Panitz1,2*, Saša Končarević3*, Ahmed Sadik1,4*, Dennis Friedel1,4, Tobias Bausbacher5, Saskia Trump6, Vadim Farztdinov3#, Sandra Schulz5, Philipp Sievers7,8, Stefan Schmidt5, Ina Jürgenson1,2, Stephan Jung3, Karsten Kuhn3, Irada Pflüger9, Suraj Sharma10, Antje Wick2, Pauline Pfänder1,4, Stefan Selzer3, Philipp Vollmuth9, Felix Sahm7,8, Andreas von Deimling7,8, Ines Heiland10, Carsten Hopf5, Peter Schulz-Knappe, Ian Pike11, Michael Platten12,13, Wolfgang Wick2,14, Christiane A. Opitz1,2 

1. DKTK Brain Cancer Metabolism Group, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
2. Department of Neurology and National Center for Tumor Diseases, Heidelberg University Hospital, 69120 Heidelberg, Germany.
3. Proteome Sciences R&D GmbH & Co. KG, Altenhöferallee 3, 60438 Frankfurt/Main, Germany.
4. Faculty of Bioscience, Heidelberg University, 69120 Heidelberg, Germany.
5. Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, 68163 Mannheim, Germany.
6. Molecular Epidemiology Unit, Berlin Institute of Health at Charité - Universitätsmedizin Berlin, 10117 Berlin, Germany.
7. Department of Neuropathology, Institute of Pathology, Heidelberg University Hospital, 69120 Heidelberg, Germany.
8. Clinical Cooperation Unit Neuropathology, German Consortium for Translational Cancer Research (DKTK), German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
9. Department of Neuroradiology, Heidelberg University Hospital, 69120 Heidelberg, Germany.
10. Department of Arctic and Marine Biology, UiT, The Arctic University of Norway, 9037 Tromsø, Norway.
11. Proteome Sciences plc, 5 Dashwood Lang Road, Bourne Business Park, Addlestone, Surrey KT15 2HJ, United Kingdom.
12. Department of Neurology, Medical Faculty Mannheim, University of Heidelberg, 68167 Mannheim, Germany.
13. DKTK Clinical Cooperation Unit Neuroimmunology and Brain Tumor Immunology, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
14. Clinical Cooperation Unit Neurooncology, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
*These authors contributed equally to this work.
#Current address: Core Facility - High-Throughput Mass Spectrometry, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health (BIH), 10117 Berlin, Germany.
§Current address: Mittelorbroich 125, 47839 Krefeld, Germany.

M2aia-Interactive, fast, and memory-efficient analysis of 2D and 3D multi-modal mass spectrometry imaging data

Journal: Gigascience, 10.1093/gigascience/giab049

J. Cordes1,2, T. Enzlein3, Ch. Marsching3, M. Hinze1, S. Engelhardt4, C. Hopf3, I. Wolf1

1Faculty of Computer Science, Mannheim University of Applied Sciences, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany.

2Medical Faculty Mannheim, University Heidelberg, Theodor Kutzer-Ufer 1-3, 68167 Mannheim, Germany.

3Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany.

4Working Group "Artificial Intelligence in Cardiovascular Medicine" (AICM), University Hospital Heidelberg, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.

MIR spectroscopy versus MALDI-ToF-MS for authenticity control of honeys from different botanical origins based on soft independent modelling by class analogy (SIMCA) – A clash of techniques?

Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, https://doi.org/10.1016/j.saa.2021.120225

R. Brendel1,2,4 , S. Schwolow1, N. Gerhardt1,5, J. Schwab4, P. Rau4, M. Oest2, S. Rohn2,3, P. Weller1

1Institute for Instrumental Analytics and Bioanalytics, Mannheim University of Applied Sciences, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany

2Institute of Food Chemistry, Hamburg School of Food Science, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany

3Department of Food Chemistry and Analysis, Institute of Food Technology and Food Chemistry, Technische Universität Berlin, TIB 4/3-1, Gustav-Meyer-Allee 25, 13355 Berlin, Germany

4Micro-Biolytics GmbH, Schelztorstraße 54, 73728 Esslingen am Neckar, Germany

5Bavarian Health and Food Safety Authority (LGL), Veterinärstr. 2, 85764 Oberschleißheim, Germany

**Editor’s Pick 2021** - A Novel Optical Tissue Clearing Protocol for Mouse Skeletal Muscle to Visualize Endplates in Their Tissue Context (Kopie 1)

Journal: Frontiers in Cellular Neuroscience, doi.org/10.3389/fncel.2019.00049

M. P. I. Williams1,*, M. Rigon1,*, T. Straka1,2,*, S. J. Hörner1,2,*, M. Thiel3, N. Gretz4,5, M. Hafner1,5, M. Reischl6, R. Rudolf1,2,5,7

1Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Mannheim, Germany

2Interdisciplinary Center for Neurosciences, Heidelberg University, Heidelberg, Germany

3Department of Anesthesiology and Surgical Intensive Care Medicine, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany

4Medical Faculty Mannheim, Medical Research Center, Heidelberg University, Mannheim, Germany

5Medical Faculty Mannheim, Institute of Medical Technology, Mannheim University of Applied Sciences, Mannheim, Germany

6Institute for Automation and Applied Informatics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany

7Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

 

**Editor’s Pick 2021**

Die Veröffentlichung wurde vom Frontiers Editorial Office als Cellular Neuropathology Editor’s Pick 2021ausgewählt. Damit zeichnet die Redaktion “Artikel mit hervorragender wissenschaftlicher Qualität aus, die repräsentativ für die Arbeit in einem bestimmten Bereich sind".

Sweet Taste Is Complex: Signaling Cascades and Circuits Involved in Sweet Sensation

Journal: Frontiers in Human Neuroscience, doi: 10.3389/fnhum.2021.667709

E. von Molitor1, K. Riedel2, M. Krohn2, M. Hafner1, R. Rudolf1,3, T. Cesetti1

1Institute of Molecular and Cell Biology, Hochschule Mannheim, Mannheim, Germany.

2BRAIN AG, Zwingenberg, Germany.

3Interdisciplinary Center for Neurosciences, Heidelberg University, Heidelberg, Germany.

Simultaneous self-optimisation of yield and purity through successive combination of inline FT-IR spectroscopy and online mass spectrometry in flow reactions (Kopie 1)

Journal: Journal of Flow Chemistry, doi.org/10.1007/s41981-021-00140-x

V. Fath1, P. Lau2, Ch. Greve2, P. Weller3, N. Kockmann4, T. Röder1

1Institute of Chemical Process Engineering, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163, Mannheim, Germany

2Merck KGaA, Frankfurter Str. 250, 64293, Darmstadt, Germany

3Institute of Instrumental Analytics and Bioanalysis, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163, Mannheim, Germany

3Department of Biochemical and Chemical Engineering, Equipment Design, TU Dortmund University, Emil-Figge-Str. 68, 44227, Dortmund, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Extracellular Matrix Components Regulate Bone Sialoprotein Expression in MDA-MB-231 Breast Cancer Cells (Kopie 1)

Journal: Cells, https://doi.org/10.3390/cells10061304

F. Keller1,2,*, R. Bruch1,*, F. Clauder3, M. Hafner1,2, R. Rudolf1,2

1Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, 68163 Mannheim, Germany

2Institute of Medical Technology, Heidelberg University and Mannheim University of Applied Sciences, 68167 Mannheim, Germany

3Immundiagnostik AG, 64625 Bensheim, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Oscillating droplet reactor – towards kinetic investigations in heterogeneous catalysis on a droplet scale (Kopie 1)

Journal: Reaction Chemestry & Engineering, doi: doi.org/10.1039/D0RE00466A

T. Klement1,*, Sch. Hanf2, F. Wolff1, N. Kockmann3, St. Schunk2, T. Röder1

1Mannheim University of Applied Sciences, Institute of Chemical, Process Engineering, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany

2hte GmbH, Kurpfalzring 104, 69123 Heidelberg, Germany

3Technische Universität Dortmund, Fakultät für Bio- und Chemieingenieurwesen, Apparatedesign, Emil-Figge-Straße 68, 44227 Dortmund, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany

Nitrogen monoxide as dopant for enhanced selectivity of isomeric monoterpenes in drift tube ion mobility spectrometry with 3 H ionization (Kopie 1)

Journal: Analytical and Bioanalytical Chemistry, doi: 10.1007/s00216-021-03306-7

R. Brendel1,*, S. Rohn2, P. Weller1

1Institute for Instrumental Analytics and Bioanalytics, Mannheim University of Applied Sciences, Paul-Wittsack-Strasse 10, 68163, Mannheim, Germany

2Hamburg School of Food Science, University of Hamburg, Grindelallee 117, 20146, Hamburg, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany

Preparation, Drug Treatment, and Immunohistological Analysis of Tri-Culture Spheroid 3D Melanoma-Like Model (Kopie 1)

Journal: Meladoma, doi.org/10.1007/978-1-0716-1205-7_13

M. Schäfer1,*, J. Klicks1,*, M. Hafner1, R. Rudolf1

1Institute of Molecular and Cell BiologyHochschule Mannheim, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Regulatory Function of Sympathetic Innervation on the Endo/Lysosomal Trafficking of Acetylcholine Receptor (Kopie 1)

Journal: Frontiers in Physiology, doi.org/10.3389/fphys.2021.626707

T. Straka1,2,3,*, Ch. Schröder4, A. Roos5,6, L. Kollipara4, A. Sickmann4,7,8, M. P. I. Williams1, M. Hafner1, M. M. Khan1,2,3*, R. Rudolf1,2,3*

1Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Mannheim, Germany

2Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe, Germany

3Interdisciplinary Center for Neurosciences, Heidelberg University, Heidelberg, Germany

4Leibniz-Institut für Analytische Wissenschaften—ISAS—e.V., Dortmund, Germany

5Department of Neuropediatrics, University Hospital Essen, Essen, Germany

6Children’s Hospital of Eastern Ontario Research Institute, Ottawa, ON, Canada

7Department of Chemistry, College of Physical Sciences, University of Aberdeen, Aberdeen, United Kingdom

8Medizinische Fakultät, Medizinische Proteom-Center (MPC), Ruhr-Universität Bochum, Bochum, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Kinetic Measurement of Acrylic Acid Polymerization at High Concentrations under Nearly Isothermal Conditions in a Pendula Slug Flow Reactor (Kopie 1)

Journal: Industrial & Engineering Chemistry Research, doi: 10.1021/acs.iecr.0c04732

T. Klement1, N. Kockmann2, Ch. Schwede3, T. Röder1

1Institute of Chemical Process Engineering, Mannheim University of Applied Sciences, Mannheim

2Faculty of Biochemical and Chemical Engineering, Laboratory of Equipment Design, TU Dortmund University, Dortmund

3BASF SE, RCP/ME-B01, Ludwigshafen am Rhein

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany 

Fast Nanoliter-Scale Cell Assays Using Droplet Microarray–Mass Spectrometry Imaging (Kopie 1)

Journal: Advanced Biology, doi.org/10.1002/adbi.202000279

C. Ramallo Guevara1, D. Paulssen2, A. Popova2, C. Hopf1, P. Levkin2

1Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences Paul-Wittsack Str. 10, 68163 Mannheim, Germany

2Karlsruhe Institute of Technology (KIT) Institute of Biological and Chemical Systems – Functional Molecular, Systems (IBCS-FMS) Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany

Volatilomic Profiling of Citrus Juices by Dual-Detection HS-GC-MS-IMS and Machine Learning—An Alternative Authentication Approach (Kopie 1)

Journal: Journal of Agricultural and Food Chemistry, doi.org/10.1021/acs.jafc.0c07447

R. Brendel1,*, S. Schwolow2, S. Rohn2, P. Weller1

1Institute for Instrumental Analytics and Bioanalytics, Mannheim University of Applied Sciences, Paul-Wittsack-Straße 10, 68163 Mannheim, Germany

2Institute of Food Chemistry, Hamburg School of Food Science, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany

*M2Aind-PhD Student, Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim/Germany